BG-GLA-NHS from New England Biolabs

Product Specs
ItemBG-GLA-NHS
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberS9151S
Quantity2 mg
ApplicationsCellular Biology
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Cell type-specific delivery by modular envelope designNat CommunAugust 23, 2023Daniel Strebinger, Chris J. Frangieh, Mirco J. Friedrich, Guilhem Faure, Rhiannon K. Macrae, Feng Zhang0.5 mg/ml, before incubation with a 40-fold molar excess of BG-GLA-NHS (New England Biolabs, Cat. # S9151S). To test different ratios of BG-GLA-NHS, 10-, 20-, 40-, or 80-fold molar excess of BG-GLA-NHS was added

(2) Dissipation and energy propagation across scales in an active cytoskeletal materialProceedings of the National Academy of SciencesApril 4, 2023Peter J. Foster, Jinhye Bae, Bezia Lemma, Juanjuan Zheng, William Ireland, Pooja Chandrakar, Rémi Boros, Zvonimir Dogic, Daniel J. Needleman, Joost J. Vlassakdescribed by Chandrakar et al. (26). BG-labeled tubulin was made by labeling tubulin using BG-GLA-NHS (NEB S9151S) using a protocol by A. Hyman et al. (47). To create BG microtubules, 44 μM tubulin containing 6% BG-labeled

(3) CLASP2 recognizes tubulins exposed at the microtubule plus-end in a nucleotide state–sensitive mannerScience AdvancesJanuary 6, 2023Wangxi Luo, Vladimir Demidov, Qi Shen, Hugo Girão, Manas Chakraborty, Aleksandr Maiorov, Fazly I. Ataullakhanov, Chenxiang Lin, Helder Maiato, Ekaterina L. Grishchukof 5′-NH2-oligos at 2 mM, 8 μl of 200 mM Hepes buffer (pH 8.5), and 12 μl of BG-GLA-NHS (catalog no. S9151S, NEB, MA, USA) prepared in dimethyl sulfoxide at 20 mM. The mixture was incubated for 30 min at 25°C,

(4) The MIDAS domain of AAA mechanoenzyme Mdn1 forms catch bonds with two different substrateseLifeFebruary 11, 2022Keith J Mickolajczyk, Paul Dominic B Olinares, Brian T Chait, Shixin Liu, Tarun M Kapoor(BG) group was covalently attached to the amino-oligonucleotides using BG-GLA-NHS (New England BioLabs S9151S) under manufacturer-suggested reaction conditions, and subsequently purified using Micro Bio-Spin P-6

(5) Tight nanoscale clustering of Fcγ receptors using DNA origami promotes phagocytosiseLifeJune 3, 2021Nadja Kern, Rui Dong, Shawn M Douglas, Ronald D Vale, Meghan A MorrisseymM. N-hydroxysuccinimide ester (BG-GLA-NHS)-functionalized benzylguanine was purchased from NEB (Cat# S9151S) and freshly reconstituted in DMSO to a final concentration of 83 mM. To functionalize the oligonucleotides

(6) Improved resolution in single-molecule localization microscopy using QD-PAINTExperimental & Molecular MedicineMarch 1, 2021Yeonho Chang, Do-Hyeon Kim, Kai Zhou, Min Gyu Jeong, Soyeon Park, Yonghoon Kwon, Triet Minh Hong, Jungeun Noh, Sung Ho Ryudocking strands (2 mM) with NH2 in HEPES buffer (200 mM, pH 8.5) were reacted with BG-GLA-NHS (20 mM, #S9151S, New England Biolabs) in anhydrous dimethyl sulfoxide (DMSO, #D8418, Sigma-Aldrich) at RT for 30 min

(7) The Tail of Kinesin-14a in Giardia Is a Dual Regulator of MotilityCurrent BiologySeptember 1, 2020Kuo-Fu Tseng, Keith J. Mickolajczyk, Guangxi Feng, Qingzhou Feng, Ethiene S. Kwok, Jesse Howe, Elisar J. Barbar, Scott C. Dawson, William O. Hancock, Weihong QiuAC215092500 Gold nanoparticles 30nm,BBI Solutions,SKU# EM.GC30 BG-GLA-NHS,New England Biolabs,Cat# S9151S Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays Branson Ultrasonics™

(8) Enhanced enzymatic activity exerted by a packed assembly of a single type of enzyme†Chemical ScienceJuly 27, 2020Huyen Dinh, Eiji Nakata, Kaori Mutsuda-Zapater, Masayuki Saimura, Masahiro Kinoshita, Takashi Moriimethods Materials The single-stranded M13mp18, restriction enzymes (NdeI and NotI) and BG-GLA-NHS (S9151S) were purchased from New England Biolabs. Purified oligonucleotide as the staple strands for DNA origami,

(9) Unique features in the intracellular transport of typhoid toxin revealed by a genome-wide screenPLOS PathogensApril 5, 2019Shu-Jung Chang, Sheng Chih Jin, Xuyao Jiao, Jorge E. Galántyphoid toxin (50 μg) was incubated for 2 hr at room temperature with BG-NHS (New England BioLabs, Cat #S9151S) (20 mM stock solution in DMSO) at a molar ratio of 1:3. The unreacted esters were quenched with 50

(10) In vitro Generation of CRISPR-Cas9 Complexes with Covalently Bound Repair Templates for Genome Editing in Mammalian CellsBIO-PROTOCOLJanuary 5, 2019Nataša Savić, Femke Cas Ringnalda, Christian Berk, Katja Bargsten, Jonathan Hall, Martin Jinek, Gerald Schwankfunctional group (Integrated DNA Technologies)Benzylguanine-GLA-NHS (New England Biolabs, catalog number: S9151S)HEPES (Sigma-Aldrich, catalog number: H4034-500G)N, N-Dimethylformamide (DMF) (Sigma-Aldrich, catalog

(11) Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repaireLifeMay 29, 2018Natasa Savic, Femke CAS Ringnalda, Helen Lindsay, Christian Berk, Katja Bargsten, Yizhou Li, Dario Neri, Mark D Robinson, Constance Ciaudo, Jonathan Hall, et al.Technologies,-,Custom DNA oligos/ '5 C6 NH2 modif. Chemical compound,BG-GLA-NHS,New England Biolabs,ID_NEB:S9151S,

(12) Single-cell mechanogenetics using monovalent magnetoplasmonic nanoparticlesNature ProtocolsSeptember 1, 2017Ji-wook Kim, Daeha Seo, Jung-uk Lee, Kaden M. Southard, Yongjun Lim, Daehyun Kim, Zev J. Gartner, Young-wook Jun, Jinwoo CheonCTG ACT GAC TG -3’ (Integrated DNA Technologies, Customized)BG-GLA-NHS (New England BioLabs, cat. no. S9151S) ▲ CRITICAL This reagent is highly sensitive to moisture. It should be stored at −20 °C.Tris(2-carboxyethyl)phosphine

(13) A DNA-Based T Cell Receptor Reveals a Role for Receptor Clustering in Ligand DiscriminationCellMarch 23, 2017Taylor MJ, Husain K, Gartner ZJ, Mayor S, Vale RD.Latrunculin A,Sigma,Cat#: L5163-100UG BC-GLA-NHS,New England Biolabs,Cat#: S9237S BG-GLA-NHS,New England Biolabs,Cat#: S9151S Puromycin dihydrochloride,Sigma,Cat#: P8833-10MG Experimental Models: Cell Lines,Experimental Models:

(14) The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transportOncotargetDecember 27, 2016Torgersen ML, Klokk TI, Kavaliauskiene S, Klose C, Simons K, Skotland T, Sandvig K.ricin and Shiga toxin Ricin and Shiga holotoxins were labeled with the SNAP-tag substrate BG-GLA-NHS (#S9151S, New England Biolabs) according to the manufacturer's instructions in molar ratios 1:1 and 3:1, respectively

(15) The mammalian dynein–dynactin complex is a strong opponent to kinesin in a tug-of-war competitionNature Cell BiologySeptember 1, 2016Vladislav Belyy, Max A. Schlager, Helen Foster, Armando E. Reimer, Andrew P. Carter, Ahmet Yildizand 2 were separately labeled with 1.25 mM BG-GLA N-Hydroxysuccinimide (NHS) (New England BioLabs, S9151S) and 1 mM alkyl chloride (AC) NHS (Promega, P6751), respectively, in a 50 mM HEPES pH 8.5 buffer containing

(16) A Universal Approach to Prepare Reagents for DNA-Assisted Protein AnalysisPLoS ONESeptember 18, 2014Junhong Yan, Gucci Jijuan Gu, Christian Jost, Maria Hammond, Andreas Plückthun, Ulf Landegren, Masood Kamali-Moghaddambifunctional cross-linker to functionalize the oligonucleotides with a benzylguanine (BG) moiety (BG-GLA-NHS (S9151S, NEB); 18 µl of a 10 mM solution BG-GLA-NHS freshly dissolved in DMSO). Excess BG-GLA-NHS was removed

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BG-GLA-NHS

BG-GLA-NHS from New England Biolabs

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New England Biolabs

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BG-GLA-NHS from New England Biolabs

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